A practical reference on PPARδ agonist: what it is, how it behaves, what the literature reports, and where the honest uncertainties sit.
This page was last updated on 2026-04-05 and is reviewed periodically as new material appears.
Cardarine has no approved therapeutic indication and is not marketed as a medicine. The World Anti-Doping Agency lists GW501516 as a prohibited substance at all times, covering both in-competition and out-of-competition periods. National laws vary: some countries treat it as an unapproved drug subject to import controls, while others have specific restrictions on sale for human consumption. It is often sold as a research chemical, a label that does not imply safety or legality. Enforcement actions have targeted online vendors and shipments.
Anti-doping laboratories identify GW501516 and related metabolites using liquid chromatography coupled with tandem mass spectrometry. Urine is the most common matrix, though blood and dried blood spots may also be analyzed. The method targets the parent compound and phase I and phase II metabolites, which extend the detection window. Because the substance is prohibited at all times, athletes can be tested outside competition. Detection limits and windows depend on the assay, sample type, and individual metabolism.
The pharmacological interest in cardarine centers on PPARδ activation and its downstream effects on lipid handling and mitochondrial function. In animal studies, PPARδ agonists have been associated with changes in exercise endurance and fatty acid utilization, though results vary by model and protocol. Human data remain sparse, and the absence of large controlled trials limits conclusions about efficacy. Researchers often describe the compound as a tool for probing PPARδ biology rather than a proven therapeutic agent.
Safety discussions about cardarine frequently cite rodent carcinogenicity findings reported in the 2000s. In those studies, treated animals developed tumors at multiple sites, leading sponsors to discontinue clinical development. The relevance of these findings to humans has not been resolved, but they are a major reason the compound is not approved. Current literature emphasizes uncertainty about long-term effects and the risks of unregulated use. Regulators and health agencies have not established a safe human exposure level.
Cardarine is a synthetic compound also known as GW501516, GW-501516, and sometimes endurobol. It was developed as a selective agonist of peroxisome proliferator-activated receptor delta, a nuclear receptor involved in fatty acid oxidation and energy metabolism. The compound was studied in preclinical models for metabolic and cardiovascular conditions, but it did not become a marketed human medicine. In regulatory and anti-doping contexts, it is treated as a prohibited substance rather than a licensed medicine.
| Property | Value | Notes |
|---|---|---|
| Regulatory status | Prohibited in sport; not approved as medicine | Listed by WADA at all times. |
| Common synonyms | GW501516, GW-501516, endurobol | Cardarine is a colloquial name. |
| Typical analytical method | LC-MS/MS | Detects parent compound and metabolites. |
| Common test matrix | Urine | Blood and dried blood spots also possible. |
| Legal classification | Varies by country | Often treated as unapproved drug or research chemical. |
Regulatory bodies treat GW501516 as a prohibited substance in competitive sport. The World Anti-Doping Agency added it to the prohibited list, and it falls under classes covering metabolic modulators and hormone-related agents. It is not approved by drug regulators for human use, and it is not a lawful dietary supplement. Products sold under the cardarine name may contain unlisted ingredients or different compounds. Because no approved product exists, quality and identity are not guaranteed by pharmaceutical manufacturing standards.
Cardarine is a common name for the investigational chemical GW501516, also written GW-1516. It was developed as a peroxisome proliferator-activated receptor delta agonist for metabolic conditions such as dyslipidemia. Early research focused on lipid handling and energy use in skeletal muscle and other tissues. The compound was never approved as a medicine. In public discussion, it is often grouped with performance-enhancing substances, although its receptor target differs from that of anabolic steroids or selective androgen receptor modulators. Regulatory and health authorities have issued warnings about its use.
GW501516 acts on PPARδ, a nuclear receptor that helps regulate fatty acid oxidation and energy homeostasis. In animal studies, activation of this receptor was associated with increased endurance and changes in lipid metabolism. Human trials examined effects on blood lipids and other metabolic markers, but the compound did not advance to approval. Rodent studies later reported tumors in multiple tissues at doses used in those experiments. Whether those findings translate to human risk remains uncertain, and the clinical relevance of the animal data is still debated.
Products sold as cardarine have been found to contain incorrect compounds, variable amounts, or no active ingredient at all. Independent testing is required to verify identity and purity. Common analytical approaches include high-performance liquid chromatography, mass spectrometry, and nuclear magnetic resonance for structural confirmation. These methods can distinguish GW501516 from related PPAR agonists and from unrelated steroids. For regulators and researchers, such verification is central to interpreting both biological results and adverse event reports.
Cardarine is prohibited in competitive sport under the World Anti-Doping Agency code, where it is classified as a metabolic modulator. It is not approved as a prescription medicine in the United States, European Union, or other major markets. Regulatory action has focused on its presence in sports and in products marketed as research chemicals. Because it has no accepted medical indication, supply is often unregulated. This status creates legal and safety uncertainties for anyone who encounters the substance.
PPARδ is a nuclear receptor that influences transcription of genes involved in fatty acid oxidation, lipid transport, and energy homeostasis. GW501516 binds and activates this receptor with high selectivity relative to PPARα and PPARγ in laboratory assays. Activation alters expression of target genes in skeletal muscle, liver, and adipose tissue in animal models. The exact clinical consequences of these changes in humans remain incompletely characterized, and observed effects in animals do not establish therapeutic benefit or safety.
Published studies have examined GW501516 in animal models of obesity, insulin resistance, and exercise endurance. Early human trials reportedly ended, and development was discontinued after preclinical findings raised concerns about cancer in some rodent studies. Regulatory agencies have not approved cardarine for any medical use. Its availability through non-pharmaceutical channels raises questions about identity, purity, and legal status that are separate from its laboratory pharmacology. Those questions are often addressed through analytical testing rather than assumptions about product labels.
Cardarine is a common name for GW501516, also GW-1516, a synthetic compound developed as a peroxisome proliferator-activated receptor delta (PPARδ) agonist. It belongs to a class of agents that modulate gene transcription related to lipid and energy metabolism. The compound was studied in preclinical and early clinical research for metabolic and cardiovascular conditions, but it did not progress to approved therapeutic use. Its name appears in fitness and sports contexts despite not being approved as a drug.
An alternative to solvent-based extraction for particulates on filters is the use of thermal extraction (TE)-GC/MS, which utilizes oven interfaced with the GC inlet to vaporize the analyte of the sample and into the GC inlet. This technique is more often used then solvent-based extraction, because of its better sensitivity, eliminates need for solvents, and can be fully automated. To increase the separation of the particles the GC can be coupled with a time of flight (TOF)-MS, which is a mass separation method that separates ions based on their size. Another method that utilizes EI is isotope ratio mass spectrometry (IR-MS) this instrumentation incorporates a magnetic sector analyzer and a faraday-collector detector array and separates ions based on their isotopic abundance. Isotopic abundance of carbon, hydrogen, nitrogen, and oxygen isotopic abundance become locally enriched or depleted through a variety of atmospheric processes. This information helps in determining the source of the aerosols and the interaction it has had. EI is a universal ionization method, but it does cause excessive fragmentation, and thus can be substituted with chemical ionization (CI) which is a much softer ionization method, and is often used to determine the molecular ion. One ionization method the utilizes CI is atmospheric-pressure chemical ionization (APCI).
== Further reading == Refrigeration volume, ASHRAE Handbook, ASHRAE, Inc., Atlanta, GA Stoecker and Jones, Refrigeration and Air Conditioning, Tata-McGraw Hill Publishers Mathur, M.L., Mehta, F.S., Thermal Engineering Vol II MSN Encarta Encyclopedia Andrew D. Althouse; Carl H. Turnquist; Alfred F. Bracciano (2003). Modern Refrigeration and Air Conditioning (18th ed.). Goodheart-Wilcox Publishing. ISBN 978-1-59070-280-2. Anderson, Oscar Edward (1972). Refrigeration in America: A history of a new technology and its impact. Kennikat Press. p. 344. ISBN 978-0-8046-1621-8. Shachtman, Tom (2000). Absolute Zero: And the Conquest of Cold. Mariner Books. p. 272. ISBN 978-0-618-08239-1. Woolrich, Willis Raymond (1967). The men who created cold: A history of refrigeration (1st ed.). Exposition Press. p. 212.
=== Tempe goreng === Perhaps the simplest and most popular way to prepare tempeh in Indonesia. The tempeh is sliced and seasoned in a mixture of ground garlic, coriander seeds and salt, and then deep fried in palm oil. The tempeh might be coated in batter prior to frying, or directly fried without any batter. Fried unbattered tempeh has a golden-brown color when done.
== Definition == The word Wagyu literally means "Japanese cattle" and does not denote a single breed. Before the modern breeds were established, Japan had regional populations of native cattle. After the Meiji Restoration in 1868, most were crossed for several generations with imported breeds, mainly British and continental European cattle. Two uncrossed native populations, the Mishima and Kuchinoshima cattle, survive and are considered to have remained free from European genetic influence. In modern Japanese breed and meat classifications, Wagyu usually means four breeds: the Japanese Black, Japanese Brown, Japanese Polled, and Japanese Shorthorn. Beginning in the late nineteenth century, these breeds developed from regional native cattle crossed with imported stock. The imported breeds used differed by breed and region. The resulting cattle were later established as distinct breeds through selection and closed breeding. After bovine spongiform encephalopathy (BSE) was first confirmed in Japan in 2001, the Beef Traceability Law was enacted in 2003. The system assigns each animal a ten-digit identification number and records its birth and movements; it applies to all cattle in Japan, not only Wagyu. This system is also used to verify the labeling requirements introduced for Wagyu beef in 2007. Under guidelines issued by the Ministry of Agriculture, Forestry and Fisheries, the designation Wagyu is recommended only for beef from cattle documented as one of the four breeds or specified crosses between them and confirmed as having been born and raised in Japan.
Sources: en.wikipedia.org
== Early life == Vincent Marks was born on 10 June 1930, in Harlesden, North West London, to Lewis and Rose (née Goldbaum) Marks, in a Jewish household. His parents ran a pub. Marks attended Tottenham Grammar School before going to study medicine on a scholarship at Brasenose College, Oxford, in 1948. He completed his training and qualified as a doctor from the St Thomas' Hospital in London, in 1954. It is noted that his interest in medicine was driven in part by his mother's insistence that their childhood home be neat and tidy for the "doctor's visit", leading him and his brother to think highly of doctors and medicine as a profession. During his time at Oxford, he was branded a communist after demanding that The Daily Worker, a newspaper mouthpiece of Communist Party of Great Britain, be introduced in the university's common rooms. He later joined the party, but left it in 1956 following the suppression of the Hungarian Uprising by the Soviet Union. In the 1980s he was a member of the Social Democratic Party (SDP).
=== Relaxin === There are three relaxin genes in humans. One type, H2, is made and secreted in the ovaries, as well as in the mammary glands. Relaxin acts via locally expressed specific receptors located on parenchyma and myoepithelial cells. It reaches peak concentrations 24-48 hours after birth and then declines.
In America, in 2009, further advanced counters were designed that included the ability to dispense hands-free – a feature that many operators had desired. This allowed pharmacies to automate their most commonly dispensed medications via calibrated cassettes. Thirty of a pharmacy's common medications would now be dispensed automatically. Another new model doubled that throughput via an enclosed robotic mechanism. Robotics had been employed in pharmacies since the mid-1990s, but later machines dispense and label filled patient vials in a comparatively tiny space (about nine square feet of floor space). These newer technologies allowed pharmacy staff to confidently dispense hundreds of prescriptions per day and still be able to manage the many functions of a busy community pharmacy. This would increase the number of patients that are able to be served each day.
== M == MALDI – Matrix-assisted laser desorption/ionization MBE – Molecular beam epitaxy MEIS – Medium energy ion scattering MFM – Magnetic force microscopy MIT – Magnetic induction tomography MPM – Multiphoton fluorescence microscopy MRFM – Magnetic resonance force microscopy MRI – Magnetic resonance imaging MS – Mass spectrometry MS/MS – Tandem mass spectrometry MSGE – Mechanically stimulated gas emission Mössbauer spectroscopy MTA – Microthermal analysis
=== Pharmacokinetics === The drug is orally active, in contrast to the poor oral bioavailability of ketamine. Its oral bioavailability is >60%. The time to peak levels of blixeprodil is 1.5 hours and its elimination half-life is 4.3 hours.
Sources: en.wikipedia.org
Thomas A. Wadden (born September 3, 1952) is an American psychologist who is known for his research on the behavioral, pharmacological, and surgical treatment of obesity. He is Professor of Psychology in Psychiatry at the Perelman School of Medicine at the University of Pennsylvania, where he served as Director of the Center for Weight and Eating Disorders for over two decades. From 2011 to 2021, he held the endowed Albert J. Stunkard Professorship in Psychiatry. Wadden has published more than 500 peer-reviewed scientific papers and edited seven volumes on obesity and eating disorders. He has contributed to national health policy on obesity management, serving on expert panels for the National Institutes of Health, the Federal Trade Commission, the Department of Veterans Affairs, and the United States House of Representatives. He is a fellow of The Obesity Society. In 2015, The Obesity Society created the Thomas A. Wadden Award for Distinguished Mentorship, recognizing his education of scientists and practitioners in the field of obesity.
The first observations and size measurements of nano-particles were made during the first decade of the 20th century. Zsigmondy made detailed studies of gold sols and other nanomaterials with sizes down to 10 nm and less. He published a book in 1914. He used an ultramicroscope that employs a dark field method for seeing particles with sizes much less than light wavelength. There are traditional techniques developed during the 20th century in interface and colloid science for characterizing nanomaterials. These are widely used for first generation passive nanomaterials specified in the next section. These methods include several different techniques for characterizing particle size distribution. This characterization is imperative because many materials that are expected to be nano-sized are actually aggregated in solutions. Some of methods are based on light scattering. Others apply ultrasound, such as ultrasound attenuation spectroscopy for testing concentrated nano-dispersions and microemulsions. There is also a group of traditional techniques for characterizing surface charge or zeta potential of nano-particles in solutions. This information is required for proper system stabilization, preventing its aggregation or flocculation. These methods include microelectrophoresis, electrophoretic light scattering, and electroacoustics. The last one, for instance colloid vibration current method is suitable for characterizing concentrated systems.
Founded in 2007 and located in Culpeper, Virginia in Northern Virginia, the National Audio-Visual Conservation Center is the Library of Congress's newest building. It was constructed out of a former Federal Reserve storage center and Cold War bunker. The campus is designed to act as a single site to store all of the library's movie, television, and sound collections. It is named in honor of David Woodley Packard, whose Packard Humanities Institute oversaw the design and construction of the facility. The centerpiece of the complex is a reproduction Art Deco movie theater that presents free movie screenings to the public on a semi-weekly basis.
=== Infertility === Infertility, not very well understood, posed serious social and cultural repercussions. The seventh-century scholar Sun Simiao is often quoted: "those who have prescriptions for women's distinctiveness take their differences of pregnancy, childbirth and [internal] bursting injuries as their basis." Even in contemporary fuke placing emphasis on reproductive functions, rather than the entire health of the woman, suggests that the main function of fuke is to produce children. Once again, the kidney visceral system governs the "source Qi", which governs the reproductive systems in both sexes. This source Qi was thought to "be slowly depleted through sexual activity, menstruation and childbirth." It was also understood that the depletion of source Qi could result from the movement of an external pathology that moved through the outer visceral systems before causing more permanent damage to the home of source Qi, the kidney system. In addition, the view that only very serious ailments ended in the damage of this system means that those who had trouble with their reproductive systems or fertility were seriously ill. According to traditional Chinese medical texts, infertility can be summarized into different syndrome types. These were spleen and kidney depletion (yang depletion), liver and kidney depletion (yin depletion), blood depletion, phlegm damp, liver oppression, and damp heat. This is important because, while most other issues were complex in Chinese medical physiology, women's fertility issues were simple.
== Prevalence == The prevalence of HLA-B27 varies markedly in the global population. In the United States, the estimated prevalence is 6-8%. 4% of North Africans, 2–9% of Chinese, and 0.1–0.5% of persons of Japanese descent possess the gene that codes for this antigen. Among the Sami in Northern Scandinavia (Sápmi), 24% of people are HLA-B27 positive, while 1.8% have associated ankylosing spondylitis, compared to 14-16% of Northern Scandinavians in general. In Finland, an estimated 14% of the population is positive for HLA-B27, while more than 95% of patients with ankylosing spondylitis and approximately 70–80% of patients with reactive arthritis have the genetic marker.
Sources: en.wikipedia.org
Legal status varies by country. It is not approved as a medicine, and it is prohibited in sport. Some jurisdictions restrict import, sale, or possession.
Laboratories use liquid chromatography-tandem mass spectrometry to detect GW501516 and its metabolites. Urine is commonly tested, and testing can occur in and out of competition.
No, cardarine is not a SARM. It is a PPARδ agonist, which acts on a different receptor. The two classes are often confused in online discussions.
Cardarine is an investigational synthetic compound that acts as a PPARδ agonist. It is also known as GW501516 and has been studied mainly in preclinical research. It is not an approved medicine.